Beyond attachment: Roles of DC-SIGN in dengue virus infection.
Identifieur interne : 000E71 ( Main/Exploration ); précédent : 000E70; suivant : 000E72Beyond attachment: Roles of DC-SIGN in dengue virus infection.
Auteurs : Ping Liu [États-Unis] ; Marc Ridilla [États-Unis] ; Pratik Patel [États-Unis] ; Laurie Betts [États-Unis] ; Emily Gallichotte [États-Unis] ; Lidea Shahidi [États-Unis] ; Nancy L. Thompson [États-Unis] ; Ken Jacobson [États-Unis]Source :
- Traffic (Copenhagen, Denmark) [ 1600-0854 ] ; 2017.
Descripteurs français
- KwdFr :
- Animaux, Cellules NIH 3T3, Cellules dendritiques (métabolisme), Dengue (métabolisme), Dengue (virologie), Endocytose (physiologie), Lectines de type C (métabolisme), Lignée cellulaire, Membrane cellulaire (métabolisme), Molécules d'adhérence cellulaire (métabolisme), Récepteurs de surface cellulaire (métabolisme), Souris, Virus de la dengue (pathogénicité).
- MESH :
- métabolisme : Cellules dendritiques, Dengue, Lectines de type C, Membrane cellulaire, Molécules d'adhérence cellulaire, Récepteurs de surface cellulaire.
- pathogénicité : Virus de la dengue.
- physiologie : Endocytose.
- virologie : Dengue.
- Animaux, Cellules NIH 3T3, Lignée cellulaire, Souris.
English descriptors
- KwdEn :
- Animals, Cell Adhesion Molecules (metabolism), Cell Line, Cell Membrane (metabolism), Dendritic Cells (metabolism), Dengue (metabolism), Dengue (virology), Dengue Virus (pathogenicity), Endocytosis (physiology), Lectins, C-Type (metabolism), Mice, NIH 3T3 Cells, Receptors, Cell Surface (metabolism).
- MESH :
- chemical , metabolism : Cell Adhesion Molecules, Lectins, C-Type, Receptors, Cell Surface.
- metabolism : Cell Membrane, Dendritic Cells, Dengue.
- pathogenicity : Dengue Virus.
- physiology : Endocytosis.
- virology : Dengue.
- Animals, Cell Line, Mice, NIH 3T3 Cells.
Abstract
Dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), a C-type lectin expressed on the plasma membrane by human immature dendritic cells, is a receptor for numerous viruses including Ebola, SARS and dengue. A controversial question has been whether DC-SIGN functions as a complete receptor for both binding and internalization of dengue virus (DENV) or whether it is solely a cell surface attachment factor, requiring either hand-off to another receptor or a co-receptor for internalization. To examine this question, we used 4 cell types: human immature dendritic cells and NIH3T3 cells expressing either wild-type DC-SIGN or 2 internalization-deficient DC-SIGN mutants, in which either the 3 cytoplasmic internalization motifs are silenced by alanine substitutions or the cytoplasmic region is truncated. Using confocal and super-resolution imaging and high content single particle tracking, we investigated DENV binding, DC-SIGN surface transport, endocytosis, as well as cell infectivity. DC-SIGN was found colocalized with DENV inside cells suggesting hand-off at the plasma membrane to another receptor did not occur. Moreover, all 3 DC-SIGN molecules on NIH3T3 cells supported cell infection. These results imply the involvement of a co-receptor because cells expressing the internalization-deficient mutants could still be infected.
DOI: 10.1111/tra.12469
PubMed: 28128492
Affiliations:
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School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Patel, Pratik" sort="Patel, Pratik" uniqKey="Patel P" first="Pratik" last="Patel">Pratik Patel</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Betts, Laurie" sort="Betts, Laurie" uniqKey="Betts L" first="Laurie" last="Betts">Laurie Betts</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Gallichotte, Emily" sort="Gallichotte, Emily" uniqKey="Gallichotte E" first="Emily" last="Gallichotte">Emily Gallichotte</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Shahidi, Lidea" sort="Shahidi, Lidea" uniqKey="Shahidi L" first="Lidea" last="Shahidi">Lidea Shahidi</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Thompson, Nancy L" sort="Thompson, Nancy L" uniqKey="Thompson N" first="Nancy L" last="Thompson">Nancy L. Thompson</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Jacobson, Ken" sort="Jacobson, Ken" uniqKey="Jacobson K" first="Ken" last="Jacobson">Ken Jacobson</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author></analytic><series><title level="j">Traffic (Copenhagen, Denmark)</title><idno type="eISSN">1600-0854</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Adhesion Molecules (metabolism)</term><term>Cell Line</term><term>Cell Membrane (metabolism)</term><term>Dendritic Cells (metabolism)</term><term>Dengue (metabolism)</term><term>Dengue (virology)</term><term>Dengue Virus (pathogenicity)</term><term>Endocytosis (physiology)</term><term>Lectins, C-Type (metabolism)</term><term>Mice</term><term>NIH 3T3 Cells</term><term>Receptors, Cell Surface (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules NIH 3T3</term><term>Cellules dendritiques (métabolisme)</term><term>Dengue (métabolisme)</term><term>Dengue (virologie)</term><term>Endocytose (physiologie)</term><term>Lectines de type C (métabolisme)</term><term>Lignée cellulaire</term><term>Membrane cellulaire (métabolisme)</term><term>Molécules d'adhérence cellulaire (métabolisme)</term><term>Récepteurs de surface cellulaire (métabolisme)</term><term>Souris</term><term>Virus de la dengue (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cell Adhesion Molecules</term><term>Lectins, C-Type</term><term>Receptors, Cell Surface</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Dendritic Cells</term><term>Dengue</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules dendritiques</term><term>Dengue</term><term>Lectines de type C</term><term>Membrane cellulaire</term><term>Molécules d'adhérence cellulaire</term><term>Récepteurs de surface cellulaire</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Dengue Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de la dengue</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Endocytose</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Endocytosis</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Dengue</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Dengue</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Mice</term><term>NIH 3T3 Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules NIH 3T3</term><term>Lignée cellulaire</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), a C-type lectin expressed on the plasma membrane by human immature dendritic cells, is a receptor for numerous viruses including Ebola, SARS and dengue. A controversial question has been whether DC-SIGN functions as a complete receptor for both binding and internalization of dengue virus (DENV) or whether it is solely a cell surface attachment factor, requiring either hand-off to another receptor or a co-receptor for internalization. To examine this question, we used 4 cell types: human immature dendritic cells and NIH3T3 cells expressing either wild-type DC-SIGN or 2 internalization-deficient DC-SIGN mutants, in which either the 3 cytoplasmic internalization motifs are silenced by alanine substitutions or the cytoplasmic region is truncated. Using confocal and super-resolution imaging and high content single particle tracking, we investigated DENV binding, DC-SIGN surface transport, endocytosis, as well as cell infectivity. DC-SIGN was found colocalized with DENV inside cells suggesting hand-off at the plasma membrane to another receptor did not occur. Moreover, all 3 DC-SIGN molecules on NIH3T3 cells supported cell infection. These results imply the involvement of a co-receptor because cells expressing the internalization-deficient mutants could still be infected.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Liu, Ping" sort="Liu, Ping" uniqKey="Liu P" first="Ping" last="Liu">Ping Liu</name></region><name sortKey="Betts, Laurie" sort="Betts, Laurie" uniqKey="Betts L" first="Laurie" last="Betts">Laurie Betts</name><name sortKey="Gallichotte, Emily" sort="Gallichotte, Emily" uniqKey="Gallichotte E" first="Emily" last="Gallichotte">Emily Gallichotte</name><name sortKey="Jacobson, Ken" sort="Jacobson, Ken" uniqKey="Jacobson K" first="Ken" last="Jacobson">Ken Jacobson</name><name sortKey="Patel, Pratik" sort="Patel, Pratik" uniqKey="Patel P" first="Pratik" last="Patel">Pratik Patel</name><name sortKey="Ridilla, Marc" sort="Ridilla, Marc" uniqKey="Ridilla M" first="Marc" last="Ridilla">Marc Ridilla</name><name sortKey="Shahidi, Lidea" sort="Shahidi, Lidea" uniqKey="Shahidi L" first="Lidea" last="Shahidi">Lidea Shahidi</name><name sortKey="Thompson, Nancy L" sort="Thompson, Nancy L" uniqKey="Thompson N" first="Nancy L" last="Thompson">Nancy L. 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